Use of the Spectral Scanning Capabilities of the PowerWaveTM 200 Microplate Spectrophotometer

Introduction The spectral analysis of samples has a number of different purposes. The most obvious is the determination of the wavelength of light that is absorbed maximally by a particular solution. Similarly, absorbance profiles can be determined in order to ascertain wavelengths, while not necessarily maximal, that provide adequate absorbance for accurate determination. Contaminants in samples can be detected by the deviation from known patterns of spectral analysis. In many cases the contaminant can be determined by the new spectral scan. Newly synthesized or unknown compounds can also be identified using a spectral analysis of that compound in solution. Here we describe some of the applications for spectral analysis of compounds in solution.